Part:BBa_K641008:Design

{Pctx}{rbs.ffGFP}

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 193
  

Design Notes

The only design considerations were tuning an rbs to the ffGFP so that expression levels could clearly be distinguished.

Source

Pctx is a promoter from a virulence island in Vibrio cholerae. The ffGFP is a widely used purely synthetic protein.